Stern-Volmer relationship

Equation used to calculate the kinetics of a photochemical intermolecular deactivation process (fluorescence and phosphorescence).

Formula

{\frac {I_{f}^{0}}{I_{f}}}=1+k_{q}\tau _{0}\cdot [{\mathrm {Q}}] with I_{f}^{0} the fluorescence rate without a quencher, I_{f} the fluorescence rate with a quencher, kq the quencher rate coefficient, τ0 the lifetime of the emissive excited state of A, without a quencher present and [Q] the concentration of the quencher.